The anticodon sequence of tRNAPylUCA from pB_PM13 was also converted to CUA following the strategy described in 2.2.5. However, since the intein is specific for Sec insertion, the codons at position 2 of sfGFP and position 204 (position 1 of the intein) had to also be switched. Primer pairs PM17/18 and PM19/20 were used to convert 2TGA to 2TAG and pB_04 to pB_04TGA, respectively.
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