Lipid Standards for Analytical Assays

The internal standards (d5-17:0/17:1/17:0) TG, 15:0 lysophosphatidylcholine (LPC), (19:0/19:0) phosphatidylethanolamine (PE), 13:0 lysophosphatidylethanolamine (LPE), d18:1/6:0 sphingomyelin (SM), (17:0/17:0) phosphatidylserine (PS), and (17:0/17:0) phosphatidylglycerol (PG) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA, 99%). A fifty-two fatty acid methyl ester (FAME) solution was obtained from NU-CHEK-PREP Co., Ltd. (Elysian, MN, USA, ≥99%). Porcine pancreatin (4 × USP, CAS: 8049-47-6) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Cholesterol standard (CAS: 57-88-5, ≥99.5%) was obtained from Beijing Dingguo Changsheng Biotechnology Co., Ltd. (Beijing, China). Sodium cholate (CAS: 361-09-1, 98%) was obtained from J&K Scientific Ltd. (Beijing, China). Silica gel G TLC plate was purchased from Bangkai (Jining, China). All analytical solvents (methyl tert-butyl ether (MTBE), CHCl_3, CH₃OH, n-hexane, etc.) used herein were high performance liquid chromatography or analytical reagent grade.

Free full text: Click here

Wu D., Zhang L., Zhang Y., Shi J., Tan C.P., Zheng Z, & Liu Y. (2023). Lipid Profiles of Human Milk and Infant Formulas: A Comparative Lipidomics Study. Foods, 12(3), 600.

Publication 2023

phosphatidylethanolamine phosphatidylserine phosphatidylglycerol

Alabaster Chcl3 Cholesterol Ester Fatty acid High performance liquid chromatography Lipids Lysophosphatidylcholine Lysophosphatidylethanolamine Methyl tert butyl ether N hexane Pancreatin Phosphatidylethanolamine Phosphatidylglycerol Phosphatidylserine Porcine Silica gel Sodium cholate Solvents Sphingomyelin

Corresponding Organization : Second Affiliated Hospital of Nanjing Medical University

Other organizations : Universiti Putra Malaysia

Top 5 similar protocols

Variable analysis

independent variables

D5-17:0/17:1/17:0 TG
15:0 lysophosphatidylcholine (LPC)
(19:0/19:0) phosphatidylethanolamine (PE)
13:0 lysophosphatidylethanolamine (LPE)
D18:1/6:0 sphingomyelin (SM)
(17:0/17:0) phosphatidylserine (PS)
(17:0/17:0) phosphatidylglycerol (PG)
Fifty-two fatty acid methyl ester (FAME) solution
Porcine pancreatin (4 × USP)
Cholesterol standard
Sodium cholate

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Fifty-two fatty acid methyl ester (FAME) solution

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!