Vero cells (ATCC CCL-81, Manassas, Virginia, United States) and Vero/hSLAM cells (ECACC 04091501, Porton Down, United Kingdom) were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Microtech, Naples, Italy) supplemented with antibiotic solution 100× (Himedia, Naples, Italy) and 10% Fetal Bovine Serum (Microtech).
HSV-1 (strain SC16), containing a lacZ gene driven by the cytomegalovirus IE-1 promoter to express beta-galactosidase, and fluorescent HSV-1, containing the GFP reporter inserted into the gene coding for the VP22 tegument protein [54 (link)], were propagated on Vero cells, as previously reported [37 (link)]. Measle virus (ATCC VR-24) was grown on VERO/hSLAM cells, while HPIV-2 (ATCC VR-92), HCoV-229E (ATCC VR-740), and SARS-CoV-2 (strain VR PV10734, kindly donated by the Lazzaro Spallanzani Hospital of Rome, Italy) and Enterovirus C (Sb-1, poliovirus Sabin strain chat, ATCC VR-1562) on Vero cell line.
HSV-1 (strain SC16), containing a lacZ gene driven by the cytomegalovirus IE-1 promoter to express beta-galactosidase, and fluorescent HSV-1, containing the GFP reporter inserted into the gene coding for the VP22 tegument protein [54 (link)], were propagated on Vero cells, as previously reported [37 (link)]. Measle virus (ATCC VR-24) was grown on VERO/hSLAM cells, while HPIV-2 (ATCC VR-92), HCoV-229E (ATCC VR-740), and SARS-CoV-2 (strain VR PV10734, kindly donated by the Lazzaro Spallanzani Hospital of Rome, Italy) and Enterovirus C (Sb-1, poliovirus Sabin strain chat, ATCC VR-1562) on Vero cell line.
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