Micro-CT Analysis of Skull Specimens

Micro-CT analyses of specimens were conducted as previously described [18 (link)]. Before micro-CT analyses, the skull specimens were collected and fixed in 4% paraformaldehyde for 1 day. Then. micro-CT images were obtained from high-resolution Inveon micro-CT (Siemens, Munich, Germany) with a resolution of 8.99 μm at 80 kV, 500 μA and an exposure time of 1500 ms. All specimens were scanned using the uniform parameters in the same container. Multimodal three-dimensional (3D) visualization software (Inveon Research Workplace; Siemens, Munich, Germany) was used to reconstruct 3D images. Bone volume (BV) and bone volume/tissue volume (BV/TV) were calculated.

Free full text: Click here

Han Y., Yang Q., Huang Y., Jia L., Zheng Y, & Li W. (2022). Long non-coding RNA SNHG5 promotes the osteogenic differentiation of bone marrow mesenchymal stem cells via the miR-212-3p/GDF5/SMAD pathway. Stem Cell Research & Therapy, 13, 130.

Publication 2022

Inveon micro-CT Inveon Research Workplace

Bone Bone tissue Micro ct Multimodal Paraformaldehyde Skull

Corresponding Organization :

Other organizations : Peking University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Bone volume (BV)
Bone volume/tissue volume (BV/TV)

control variables

Uniform parameters for scanning all specimens
All specimens scanned in the same container

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!