Isolation and Characterization of Ginger Compounds

Ginger extract, which contains 20% gingerols and shogaols, was obtained from Sabinsa Corporation (Piscataway, NJ). RP C-18 silica gel, silica gel, Sephadex LH-20 gel, TLC plates (250 µm thickness, 2–25 µm particle size), CD3OD, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma (St. Louis, MO). HPLC-grade solvents and other reagents were obtained from VWR Scientific (South Plainfield, NJ). HPLC-grade water was prepared using a Millipore Milli-Q purification system (Bedford, MA). H-1299 human lung cancer cells, HCT-116 human colon adenocarcinoma cells, and RAW 264.7 murine macrophages were obtained from American Type Tissue Culture (Manassas, VA, USA).

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Sang S., Hong J., Wu H., Liu J., Yang C.S., Pan M.H., Badmaev V, & Ho C.T. (2009). Increased Growth Inhibitory Effects on Human Cancer Cells and Anti-Inflammatory Potency of Shogaols from Zingiber officinale Relative to Gingerols. Journal of agricultural and food chemistry, 57(22), 10645-10650.

Publication 2009

Bromide Cells Colon adenocarcinoma Diphenyl Ginger extract Gingerols Hct 116 cells Hplc Human Lung cancer Macrophages Murine Sephadex lh 20 Silica gel Solvents Tetrazolium Tissue type

Corresponding Organization :

Other organizations : North Carolina Central University, Rutgers, The State University of New Jersey, National Kaohsiung Marine University, Sabin Vaccine Institute

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Variable analysis

independent variables

Ginger extract, which contains 20% gingerols and shogaols

dependent variables

Not explicitly mentioned

control variables

RP C-18 silica gel
Silica gel
Sephadex LH-20 gel
TLC plates (250 µm thickness, 2–25 µm particle size)
CD3OD
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)
HPLC-grade solvents and other reagents
HPLC-grade water prepared using a Millipore Milli-Q purification system
H-1299 human lung cancer cells
HCT-116 human colon adenocarcinoma cells
RAW 264.7 murine macrophages

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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