Ae. aegypti, An. gambiae and Ge. atropalpus were conventionally reared in the same insectary at 28° C, ~60% relative humidity (RH), and 16 h light: 8 h dark photoperiod (Riehle & Brown 2002 (link)). Larvae were fed a standard diet consisting of finely ground rat chow (Purina): lactalbumin: brewers yeast (1:1:1) in open aluminum rearing pans containing distilled water produced in the laboratory. Pupae in water from the larval rearing pans were transferred to plastic cages where adults emerged and commonly imbibed water from rearing pans. After emergence, conventionally reared adults were provided 10% sucrose in water ad libitum. Ge. atropalpus females thereafter laid a first clutch of eggs on filter paper. Adult female Ae. aegypti and An. gambiae were blood fed two days post-emergence on an anesthetized rat until engorged. Females then laid a clutch of eggs approximately 36 h later on filter paper. Eggs from each species were stored in humidified containers at room temperature until needed.