Transcriptional Analysis of Cell Lines

All cell lines were treated with 0.2 μM IDR or 0.1 μM Am80 for 4 h. Total RNA was isolated using a high-purity RNA isolation kit (Roche Diagnostics, Mannheim, Germany). Reverse transcription polymerase chain reaction (RT-PCR) was carried out using a Titan One Tube RT-PCR kit (Roche Diagnostics) according to the manufacturer’s instructions. cDNA derived from each cell line was amplified by 28 PCR cycles. The relative signal intensity of bands was determined and standardized using imaging software (Scion, Frederick, MD, USA) as described previously [12 (link), 13 (link)].

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Tsunaka M., Shinki H, & Koyama T. (2017). Cell-based evaluation of changes in coagulation activity induced by antineoplastic drugs for the treatment of acute myeloid leukemia. PLoS ONE, 12(4), e0175765.

Publication 2017

high-purity RNA isolation kit Titan One Tube RT-PCR kit

Am80 Cdna Cell line Diagnostics Isolation Rt pcr

Corresponding Organization : Tokyo Medical and Dental University

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Variable analysis

independent variables

Treatment with 0.2 μM IDR
Treatment with 0.1 μM Am80

dependent variables

Relative signal intensity of bands

control variables

Cell lines
RNA isolation method
Reverse transcription polymerase chain reaction (RT-PCR) protocol
Number of PCR cycles (28)

controls

No positive or negative controls were explicitly mentioned.

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