Western Blot Analysis of Apoptosis Regulators

Western blot analysis was performed by standard methods as previously described29 (link). The antibodies against BCL-2, BCL-XL, MCL-1, BAX, BAK, cleaved caspase 3, cytochrome c, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), Akt, p-Akt, and β-tubulin were purchased from Cell Signaling Technology. The antibodies against PARP, BIM, p53, MDM2, and PUMA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibody against BTK was purchased from ImmunoWay Biotechnology (JiangSu, China). The secondary anti-mouse and anti-rabbit antibodies were purchased from Santa Cruz Biotechnology. Antigen–antibody complexes were detected using Bio-Rad Clarity western ECL substrate, and protein level was quantified by ImageJ (Bio-Rad Laboratory, Hercules, CA, USA).

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Luo Q., Pan W., Zhou S., Wang G., Yi H., Zhang L., Yan X., Yuan L., Liu Z., Wang J., Chen H., Qiu M., Yang D, & Sun J. (2020). A Novel BCL-2 Inhibitor APG-2575 Exerts Synthetic Lethality With BTK or MDM2-p53 Inhibitor in Diffuse Large B-Cell Lymphoma. Oncology Research, 28(4), 331-344.

Publication 2020

BCL-2 BCL-XL MCL-1 BAX cleaved caspase 3 cytochrome c glyceraldehyde 3-phosphate dehydrogenase (GAPDH), p-Akt β-tubulin secondary anti-mouse and anti-rabbit antibodies Clarity western ECL substrate ImageJ

Anti antibodies Antibodies Antibody Antigen antibody complexes Bcl 2 Caspase 3 Cytochrome c Glyceraldehyde 3 phosphate dehydrogenase Mdm2 Mouse Protein Puma Rabbit Tubulin Western blot analysis

Corresponding Organization :

Other organizations : Sun Yat-sen University, Sun Yat-sen University Cancer Center, Peking University Shenzhen Hospital, Qinghai Red Cross Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein levels of BCL-2, BCL-XL, MCL-1, BAX, BAK, cleaved caspase 3, cytochrome c, GAPDH, Akt, p-Akt, β-tubulin, PARP, BIM, p53, MDM2, and PUMA

control variables

Western blot analysis performed by standard methods as previously described29
Antibodies purchased from Cell Signaling Technology, Santa Cruz Biotechnology, and ImmunoWay Biotechnology
Secondary anti-mouse and anti-rabbit antibodies purchased from Santa Cruz Biotechnology
Antigen–antibody complexes detected using Bio-Rad Clarity western ECL substrate
Protein level quantified by ImageJ (Bio-Rad Laboratory)

controls

None explicitly mentioned

Annotations

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