As previously described (73 (link), 89 (link)–91 (link), 93 (link), 94 (link), 96 (link)–98 (link)), eyes were harvested from euthanized mice at specific time points, homogenized in 400 μl PBS with sterile 1-mm glass beads (BioSpec Products, Inc., Bartlesville OK), serially diluted 10-fold in PBS, and plated onto BHI agar plates.
For in vivo bacterial growth analysis at different time points, experimental endophthalmitis was induced by intravitreally injecting approximately 100 CFU WT B. thuringiensis in 0.5 μl BHI into the right eyes of mice. At 4 h postinfection, one group of infected eyes was treated with OxPAPC, and another group served as the untreated control. At 2 h intervals thereafter, eyes were harvested for quantitation of intraocular bacterial growth (73 (link), 89 (link)–91 (link), 93 (link), 94 (link), 96 (link)–98 (link)).
For in vivo bacterial growth analysis at different time points, experimental endophthalmitis was induced by intravitreally injecting approximately 100 CFU WT B. thuringiensis in 0.5 μl BHI into the right eyes of mice. At 4 h postinfection, one group of infected eyes was treated with OxPAPC, and another group served as the untreated control. At 2 h intervals thereafter, eyes were harvested for quantitation of intraocular bacterial growth (73 (link), 89 (link)–91 (link), 93 (link), 94 (link), 96 (link)–98 (link)).
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