24 hours after polarization, hMDM phagocytosis of S. aureus and Zymosan A pHrodo Red Bioparticles (Thermo Fisher Scientific) was measured as described previously [29 (link)], with a Bioparticle incubation time of 2 hours. Fluorescence in each well was quantified using a CLARIOstar plate reader (BMG Labtech).
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