Human umbilical vein endothelial cells (HUVECs) were provided by The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences and maintained at 37˚C in a humidified incubator with 5% CO2. HUVECs were cultured in F12K medium (Boster Biological Technology Co., Ltd.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.), 100 µg/ml streptomycin and 100 IU/ml penicillin (Boster Biological Technology Co., Ltd.). HUVECs were treated with 100 µg/ml oxidized low-density lipoprotein (ox-LDL; UnionBiol Biotechnologies Co., Ltd.) for 24 h at 37˚C to simulate atherosclerosis, as previously described (20 (link)).
Protocol detail
Culturing HUVECs for Atherosclerosis Modeling
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Atherosclerosis
Biological
Cell type
Chinese
Cultured medium
Human umbilical vein endothelial cells
Ox ldl
Penicillin
Streptomycin
Corresponding Organization :
Other organizations : Harbin Medical University, First Affiliated Hospital of Harbin Medical University
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Variable analysis
independent variables
- Oxidized low-density lipoprotein (ox-LDL) treatment
- Not explicitly mentioned
- Incubation temperature (37°C)
- Incubation atmosphere (5% CO2)
- Cell type (Human umbilical vein endothelial cells, HUVECs)
- Cell culture medium (F12K medium with 10% FBS, 100 µg/ml streptomycin and 100 IU/ml penicillin)
- No positive or negative controls were explicitly mentioned in the provided information.
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