Asthma was induced in mice as described previously.21 (link) In brief, mice were randomly divided into four groups, namely, PBS, OVA, OVA/PBS, and OVA/SJMHE1 group. On days 0, 7, and 14, each mouse in PBS group was immunized by intraperitoneal injection of 200 µL of PBS, while the mice in the other groups were immunized by 50 µg OVA (Sigma-Aldrich, Steinheim, Germany) and 2 mg of 10% aluminum hydroxide gel in PBS. On days 14 and 21, mice in OVA/PBS and OVA/SJMHE1 groups were separately treated with PBS and SJMHE1 (10 µg) emulsified with incomplete Freund’s adjuvant (Sigma, Poole, UK) as described previously.21 (link) On days 21–28, the mice were challenged in the form of atomization with OVA (2%) or PBS as previously described.21 (link) All the mice were sacrificed on the day 29 to evaluate airway inflammation and immune response.