Western Blot Analysis of Circadian Proteins
Corresponding Organization : Hebrew University of Jerusalem
Variable analysis
- Lysis buffer
- Protein levels of AMPK, pAMPK, BMAL1, pBMAL1, S6, phosphorylated S6, FAS, mTOR, CRY1, and CLOCK
- 10% SDS polyacrylamide gel
- Nitrocellulose membranes
- Antibodies against AMPK, pAMPK, BMAL1, pBMAL1, S6, phosphorylated S6, FAS, mTOR, CRY1, and CLOCK
- Horseradish peroxidase-conjugated secondary antibody
- Anti-mouse antibody for actin (loading control)
- Enhanced chemiluminescence for detection
Annotations
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