Western Blot Analysis of Cell Signaling Proteins

Western blot analysis was performed as described^{11 (link)} with nitrocellulose membranes probed for p16 (m156, 1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), phosphorylated p53 (9284, 1:2000, Cell Signaling, Danvers, MA, USA), total p53 (CM5, 1:2000, Leika, Biosystems, Richmond, IL, USA), phospho-histone γ-H2AX (1:1000, Upstate, Cell Signaling Solutions, Temecula, CA, USA), p19^ARF (sc-32748, 1:500, Santa Cruz Biotechnology), TUBA1B (DM 1A, 1:2500, Sigma-Aldrich Chemical Company, St Louis, MO, USA), GAPDH (8884, 1:10000, Cell Signaling) and/or ACTB (A5060, 1:1000, Sigma-Aldrich Chemical Company).

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Sen M., Akeno N., Reece A., Miller A.L., Simpson D.S, & Wikenheiser-Brokamp K.A. (2017). p16 controls epithelial cell growth and suppresses carcinogenesis through mechanisms that do not require RB1 function. Oncogenesis, 6(4), e320-.

Publication 2017

m156 p19ARF (sc-32748, GAPDH (8884

Gapdh Histone h2ax Membranes Nitrocellulose P19arf Western blot analysis

Corresponding Organization :

Other organizations : Cincinnati Children's Hospital Medical Center

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Variable analysis

independent variables

Nitrocellulose membranes probed for p16
Phosphorylated p53
Total p53
Phospho-histone γ-H2AX
P19^ARF
TUBA1B
GAPDH

dependent variables

Western blot analysis

control variables

Western blot analysis was performed as described in the referenced study (link provided)

controls

No specific positive or negative controls were mentioned in the provided information.

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