Histomorphological Analysis of Rat Organs

Mammary glands, uterus, vagina, kidneys, and liver were dehydrated by a series of ethanol solution, embedded in paraffin blocks before cutting into 5 μm sections, and stained with hematoxylin and eosin as previously reported by Zingue et al. [9 (link), 15 (link)]. Histomorphological changes were determined under an Axioskop 40 microscope connected to a computer where the image was transferred using MRGrab1.0 and Axio Vision 3.1 software (Zeiss Hallbergmoos, Germany). Atlas and histologic classification of tumors of rat mammary gland from Russo and Russo [16 ] were used in this study.

Free full text: Click here

Zingue S., Kamga Silihe K., Fouba Bourfane I., Boukar A., Tueche A.B., Njuh A.N, & Njamen D. (2019). Potential of Regular Consumption of Cameroonian Neem (Azadirachta indica L.) Oil for Prevention of the 7,12-Dimethylbenz(a)anthracene-Induced Breast Cancer in High-Fat/Sucrose-Fed Wistar Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 2031460.

Publication 2019

Axioskop 40 microscope MRGrab1.0 Axio Vision 3.1

Before Dehydrated ethanol Embedded paraffin Eosin Kidneys Liver Mammary gland tumors Mammary glands Microscope Uterus Vagina Vision

Corresponding Organization : Université de Yaoundé I

Top 5 similar protocols

Variable analysis

independent variables

Dehydration by a series of ethanol solutions
Embedding in paraffin blocks
Cutting into 5 μm sections
Staining with hematoxylin and eosin

dependent variables

Histomorphological changes

control variables

Mammary glands, uterus, vagina, kidneys, and liver tissues
Axioskop 40 microscope
MRGrab1.0 and Axio Vision 3.1 software
Atlas and histologic classification of tumors of rat mammary gland from Russo and Russo [16]

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!