Array Comparative Genomic Hybridization Analysis

Array Comparative Genomic Hybridization analysis was performed using SurePrint G3 Human CGH plus SNP Microarray following the manufacturer’s instructions (Agilent Technologies, Palo Alto, CA, USA). Genomic DNA was extracted using Wizard Genomic DNA Purification Kit (Promega TM, Mannheim, Germany) or GenElute Blood Genomic DNA kit (Sigma, Darmstadt, Germany), according to the manufacturer’s instructions. DNA concentration was determined on a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Berlin, Germany). DNA control reference was provided by Agilent. The array was scanned at 3-µm resolution using Agilent microarray scanner and analyzed using Agilent microarray scanner and analyzed using Feature Extraction v10.7 in order to read scanner image and Genomic Workbench v7.0 (Agilent Technologies, Palo Alto, CA, USA) to analyze copy number variations. Significant chromosomal aberration was determined using the algorithm ADM-2 (threshold, 5.0; absolute minimum average log2 ratio, 0.20; with at least three or more consecutive probe sets; see more detailed in [29 (link)]).