Investigating Drosophila DNA Damage Repair

The following fly stocks were used in this study: UAS-DDB1, UAS-DDB1^RNAi-Res, ddb1^HK-2-3, ddb1^W197 (generated in this study), ddb1^5-1, cul4^G1-3, cul4^JJ11, UAS-Flag-Cul4, UAS-Flag-Cul4^KR (CT Chien); mahj¹ [36 (link)]; UAS-Myc-Mahj, UAS-Myc-Mahj^R1120/1123E (this study). The following fly strains were obtained from BDSC: β-gal^RNAi (#50680), UAS-CD8-GFP (#32186), wts^RNAi (#34064), mahj^RNAi1 (#34912) and UAS-Yki^S168A (#28818); RNAi lines including ddb1^RNAi (#44974) and cul4^RNAi1 (#105668), cul4^RNAi2 (#44829) and mahj^RNAi2 (#110669) were obtained from the VDRC. Df(2R) XE2900 (#108418) is from the Kyoto Drosophila Genomics and Genetic Resource.
NSC drivers included insc-Gal4 (BDSC#8751; 1407-Gal4) or insc-Gal4, tub-Gal80^ts. Glial driver was repo-Gal4 (BDSC# 7145). Ubiquitous driver was tub-Gal4 (BDSC#5138). UAS-Dcr2 (BDSC#24650) was used together with various RNAi stocks. ddb1 and mahj RNAi knockdown efficiency was verified by immunostaining of anti-DDB1 and anti-Mahj antibodies in larval brains.
All experiments with mutants were carried out at 25 °C, and experiments for RNAi knockdown or overexpression were performed at 29 °C.

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Ly P.T., Tan Y.S., Koe C.T., Zhang Y., Xie G., Endow S., Deng W.M., Yu F, & Wang H. (2019). CRL4Mahj E3 ubiquitin ligase promotes neural stem cell reactivation. PLoS Biology, 17(6), e3000276.

Publication 2019

insc-Gal4 repo-Gal4 tub-Gal4 UAS-Dcr2

Anti antibodies Brains Dcr2 Ddb1 Drosophila Glial Larval Rnai Strains

Corresponding Organization : National University of Singapore

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Variable analysis

independent variables

UAS-DDB1
UAS-DDB1^RNAi-Res^
Ddb1^HK-2-3^
Ddb1^W197^ (generated in this study)
Ddb1^5-1^
Cul4^G1-3^
Cul4^JJ11^
UAS-Flag-Cul4
UAS-Flag-Cul4^KR^
Mahj^1^
UAS-Myc-Mahj
UAS-Myc-Mahj^R1120/1123E^
β-gal^RNAi^ (#50680)
Wts^RNAi^ (#34064)
Mahj^RNAi1^ (#34912)
UAS-Yki^S168A^ (#28818)
Ddb1^RNAi^ (#44974)
Cul4^RNAi1^ (#105668)
Cul4^RNAi2^ (#44829)
Mahj^RNAi2^ (#110669)
Df(2R) XE2900 (#108418)
Insc-Gal4 (BDSC#8751; 1407-Gal4)
Insc-Gal4, tub-Gal80^ts^
Repo-Gal4 (BDSC# 7145)
Tub-Gal4 (BDSC#5138)
UAS-Dcr2 (BDSC#24650)

dependent variables

Not explicitly mentioned

control variables

UAS-CD8-GFP (#32186)
β-gal^RNAi^ (#50680)
All experiments with mutants were carried out at 25 °C
Experiments for RNAi knockdown or overexpression were performed at 29 °C

positive controls

None specified

negative controls

None specified

Annotations

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