Establishment and Characterization of Murine Ovarian Cancer Models

Female C57BL/6 mice were obtained from Charles River Laboratories (Wilmington, MA, USA). B6.129S(C)-Batf3^tm1Kmm/J mice were purchased from The Jackson Laboratory (Sacramento, CA, USA). Experimental procedures were performed in compliance with protocols approved by the Institutional Animal Care and Use Committee of the Roswell Park Comprehensive Cancer Center (RPCCC, Buffalo, NY, USA). The parental ID8 mouse ovarian epithelial cell line, derived from spontaneous malignant transformation of C57BL/6 MOSE cells,⁶⁰ (link) and its metastatic variant ID8-T were established in our laboratory at the RPCCC.⁶ (link) Human HuTK⁻ 143 fibroblasts, human cervical carcinoma HeLa cells, and the African green monkey cell line CV-1 were obtained from the American Type Culture Collection (Manassas, VA, USA).