Mosquito Cell Lines for RNAi Studies

Aag2 (RRID:CVCL_Z617), AF05 (RRID:CVCL_A8M5) and AF319 (RRID:CVCL_A8M6) cells (Ae. aegypti derived), U4.4 (RRID:CVCL_Z820) and C6/36 (RRID:CVCL_Z230) cells (Ae. albopictus derived) were used for transfections. AF319 cells are Dcr-2 CRISPR knockout from the AF05 single cell derived parental cell line [20 (link)–22 (link)], while C6/36 cells have a non-functional Dcr-2 [23 (link)–25 (link)]. All mosquito cell lines were grown in Leibovitz-15 media (Gibco) supplemented with 10% fetal bovine serum (Labtech), 100 I.U./ml penicillin with 100μg/ml streptomycin (Gibco), and 10% tryptose phosphate broth (Gibco) at 28°C without CO2 or humidity control.

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Tng P.Y., Carabajal Paladino L.Z., Anderson M.A., Adelman Z.N., Fragkoudis R., Noad R, & Alphey L. (2022). Intron-derived small RNAs for silencing viral RNAs in mosquito cells. PLoS Neglected Tropical Diseases, 16(6), e0010548.

Publication 2022

Leibovitz-15 media fetal bovine serum penicillin streptomycin tryptose phosphate broth

Cell lines Cells Crispr Fetal bovine serum Humidity Mosquito Parental Penicillin Phosphate Streptomycin Transfections Tryptose

Corresponding Organization : The Pirbright Institute

Other organizations : Royal Veterinary College, Texas A&M University

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Variable analysis

independent variables

Cell lines used for transfections: Aag2 (RRID:CVCL_Z617), AF05 (RRID:CVCL_A8M5), AF319 (RRID:CVCL_A8M6) (Ae. aegypti derived), U4.4 (RRID:CVCL_Z820), C6/36 (RRID:CVCL_Z230) (Ae. albopictus derived)

dependent variables

Not explicitly mentioned

control variables

All mosquito cell lines were grown in Leibovitz-15 media (Gibco) supplemented with 10% fetal bovine serum (Labtech), 100 I.U./ml penicillin with 100μg/ml streptomycin (Gibco), and 10% tryptose phosphate broth (Gibco) at 28°C without CO2 or humidity control

controls

Positive control: None mentioned
Negative control: None mentioned
AF319 cells are Dcr-2 CRISPR knockout from the AF05 single cell derived parental cell line, while C6/36 cells have a non-functional Dcr-2

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