SAHA Otoprotection Against Noise Exposure

Mice were injected intraperitoneally with suberoylanilide hydroxamic acid (SAHA) (Selleckchem, TX), (100 mg/kg body weight) dissolved in DMSO (MilliporeSigma, MA), or with DMSO alone (vehicle) 3 days before exposure to noise and 2 h after the end of the noise exposure. The SAHA dosing amount was based on a previous publication using SAHA as an otoprotective agent, where the authors tried different dose concentrations of SAHA and reported a 100 mg/kg dose to be most efficacious without cytotoxicity [12 (link)]. Because studies vary in their dosing regimen for SAHA, the frequency of the dosing was based on the published literature with minor modifications [14 (link), 31 (link)].