For apoptosis assay, cells were treated according to the indicated conditions, then collected and washed twice using 4 °C PBS. An Annexin V-FITC/PI-containing apoptosis detection kit (KGA108, KeyGEN BioTECH, Nanjing, China) was used to stain the apoptotic cells according to the instruction. Flow cytometry was performed to determine the apoptotic rate from three independent experiments.
Analyzing Cell Cycle and Apoptosis by Flow Cytometry
For apoptosis assay, cells were treated according to the indicated conditions, then collected and washed twice using 4 °C PBS. An Annexin V-FITC/PI-containing apoptosis detection kit (KGA108, KeyGEN BioTECH, Nanjing, China) was used to stain the apoptotic cells according to the instruction. Flow cytometry was performed to determine the apoptotic rate from three independent experiments.
Corresponding Organization :
Other organizations : First People's Hospital of Foshan, Guangzhou Medical University
Variable analysis
- Importin-7 siRNAs
- Cell cycle distribution
- Apoptosis
- Cells were digested and washed twice with 4 °C PBS
- Cells were fixed with a mixture containing 2 ml cold 70% ethanol solution and 500 μl PBS at 4 °C overnight
- Cells were prepared into a single cell suspension using a Propidium Iodide (PI)-containing cell cycle detection kit
- Cells were stained with Annexin V-FITC/PI-containing apoptosis detection kit
- No positive or negative controls were explicitly mentioned.
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