Comparative Analysis of FC Measurement Kits

FC levels were determined with the same fecal sample using each of the following four kits, according to the protocol of manufacturer. Those included three standard kits; EliA^® Calprotectin 2 (Thermo Fisher Scientific, Sweden), PhiCal^® Calprotectin ELISA (Immundiagnostik, Germany)^{49 (link)}, and Buhlmann-E (BÜHLMANN fCAL^® ELISA, Bühlmann, Switzerland), and also the new BÜHLMANN fCAL^® turbo kit (Bühlmann, Switzerland)^{30 (link)}. All samples were measured in duplicate under the same pre-analytical conditions, with the mathematical mean used to reflect any imprecision for the patient samples. The characteristics of each kit are shown in Table S1. PhiCal assays were performed by SRL Inc. (Tokyo, Japan) as part of our previous prospective study, while the others included in the present study were performed by Nissui Pharmaceutical Co., Ltd. (Tokyo, Japan), after receiving the preserved fecal samples.

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Oka A., Kawashima K., Kishimoto K., Kotani S., Fukunaga M., Fukuba N., Mishima Y., Oshima N., Ishimura N., Awoniyi M, & Ishihara S. (2024). Validation of rapid fecal calprotectin assay using particle enhanced turbidimetric immunoassay for inflammatory bowel disease. Scientific Reports, 14, 1653.

Publication 2024

EliA® Calprotectin 2

Corresponding Organization :

Other organizations : Shimane University, Cleveland Clinic Lerner College of Medicine, Shimane University Hospital

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Variable analysis

independent variables

Kit type: EliA® Calprotectin 2 (Thermo Fisher Scientific, Sweden), PhiCal® Calprotectin ELISA (Immundiagnostik, Germany), Buhlmann-E (BÜHLMANN fCAL® ELISA, Bühlmann, Switzerland), and BÜHLMANN fCAL® turbo kit (Bühlmann, Switzerland)

dependent variables

FC (fecal calprotectin) levels

control variables

Fecal sample
Pre-analytical conditions
Duplicate measurements
Mathematical mean for patient samples

positive controls

None specified

negative controls

None specified

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