Endothelial Differentiation of Cardiac Stem Cells

For endothelial differentiation, CSC were plated at a density of 50.000 cells/well into 6-well culture plated coated with Endothelial Cell Attachment Factor (Sigma-Aldrich), in EndoGRO (Merck Millipore) and maintained in hypoxia (1% O₂ and 5% CO₂) in hypoxia chambers (Stem Cells Technologies) for 14 days, as previously described [31 (link)]. CSC-derived TEC were maintained in culture for further 20 passages without observing any loss of phenotype.

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Brossa A., Buono L, & Bussolati B. (2018). Effect of the monoclonal antibody TRC105 in combination with Sunitinib on renal tumor derived endothelial cells. Oncotarget, 9(32), 22680-22692.

Publication 2018

Endothelial Cell Attachment Factor EndoGRO

Endothelial Endothelial cell Hypoxia Phenotype Stem cells

Corresponding Organization : University of Turin

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Variable analysis

independent variables

Cell seeding density (50,000 cells/well)
Culture substrate (Endothelial Cell Attachment Factor coated)
Culture medium (EndoGRO)
Oxygen tension (1% O2)

dependent variables

Endothelial differentiation of CSC
Maintenance of phenotype in CSC-derived TEC for 20 passages

control variables

Culture conditions (maintained in hypoxia chambers)
Culture duration (14 days for endothelial differentiation, further 20 passages for maintenance)

controls

No positive or negative controls were explicitly mentioned.

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