Immunoassay Techniques for Protein Detection

Immunoperoxidase, immunofluorescence stain, and Western blot were performed as previously described.^{17 (link),18 (link)} The following antibodies were used: polyclonal anti-C4d (American Research Products); polyclonal anti-CD3 (Abcam); anti-CD68 (clone ED1, Biorad); anti-PAX5 (clone A-11, Santa Cruz); and anti-GAPDH (clone 6C5, Abcam).

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Panzer S.E., Wilson N.A., Verhoven B.M., Xiang D., Rubinstein C.D., Redfield RR I.I.I., Zhong W, & Reese S.R. (2018). Complete B Cell Deficiency Reduces Allograft Inflammation and Intragraft Macrophages a Rat Kidney Transplant Model. Transplantation, 102(3), 396-405.

Publication 2017

anti-CD68 (clone ED1, anti-GAPDH (clone 6C5,

Anti cd3 Antibodies Clone Gapdh Immunofluorescence Immunoperoxidase Pax5 Stain Western blot

Corresponding Organization : University of Wisconsin–Madison

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Variable analysis

independent variables

Immunoperoxidase stain
Immunofluorescence stain
Western blot

dependent variables

Protein expression levels

control variables

Antibodies used: polyclonal anti-C4d, polyclonal anti-CD3, anti-CD68 (clone ED1), anti-PAX5 (clone A-11), anti-GAPDH (clone 6C5)

controls

Positive controls: Not specified
Negative controls: Not specified

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