Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed as previously described (Massó-Vallés et al. 2022 (link)). Briefly, to mimic the microarrays conditions, A375 and SkMel147 Omomyc-RFP cells were treated with 1 μg/mL doxycycline for 6, 12, and 24 h and for 4 d or left untreated. RNA was then extracted with TRIzol and quantified using NanoDrop. Equal amounts of RNA were reverse-transcribed to generate cDNA using iScript reverse transcription Supermix for qRT-PCR (Bio-Rad). SYBR Green qRT-PCR analysis was then performed on these cDNA samples with PerfeCTa SYBR Green FastMix and Low Rox (Quantabio) using the QuantStudio 6 FLEX system (Applied Biosystems). The data thus obtained were analyzed following the comparative (ΔΔCt) method described by Livak and Schmittgen (2001) (link). Analysis of relative gene expression data was performed using real-time quantitative PCR and the 2−ΔΔC(T) method (Livak and Schmittgen 2001 (link)). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and β-Tubulin were used as housekeeping genes. Sequences of primers used are listed in Supplemental Table S12.