Adoptive transfers and infections were performed as described (Smith et al. 2014 (link)). Briefly, CD8+ T cells were isolated from congenically marked adult and neonatal mice, mixed at a 1:1 ratio, and 2 × 104 cells were transferred intravenously into Ly5.2 recipients. The next day, recipients were intravenously infected with Lm-gB (5 × 103 CFU). Effector CD8+ T cells from recipient spleens were isolated with anti-CD8 beads (Miltenyi Biotec) and then sorted via congenic marker expression on a FACS Aria (BD Biosciences).
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Adoptive T Cell Transfer and Infection
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Corresponding Organization : Cornell University
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Variable analysis
independent variables
- Adoptive transfers of CD8+ T cells (1:1 ratio of adult and neonatal cells)
- Infection with Lm-gB (5 × 10^3 CFU)
- Effector CD8+ T cells from recipient spleens
- Ly5.2 recipients
- Transfer of 2 × 10^4 cells intravenously
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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