Live/Dead Cell Viability Assay
Corresponding Organization :
Other organizations : University of Bristol, University Of Bristol Dental Hospital
Variable analysis
- Culturing duration (1 day or 7 days)
- Cell viability (Calcein AM staining)
- Cell death (Ethidium homodimer-1 staining)
- Bioprinted constructs
- Expansion media
- PBS (pre-warmed to 37 °C)
- Staining solution (Calcein AM and ethidium homodimer-1 in PBS)
- Incubation conditions (30 min, 37 °C, wrapped in foil)
- Widefield microscope setup (Calcein AM filter = GFP, ethidium homodimer-1 filter = Texas Red, Leica DMI6000 inverted epifluorescence microscope, Leica LASX live cell imaging workstation, Photometrics Prime 95B sCMOS camera)
Annotations
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