Isolation and Characterization of Bacteriophage PBPA90

Escherichia coli ATCC8739 was purchased from the American Type Culture Collection. Adherent invasive E. coli (AIEC) strains were kindly gifted by Professor Christel Neut (Rahmouni et al., 2018 (link)). All strains were cultured in Luria-Bertani (LB) media. Bacteriophage PBPA90 infecting P. aeruginosa was obtained from the Bacteriophage Bank of Korea. The bacteriophage was isolated from Opo Wastewater Facility in Gwangju, Gyeonggi-do, South Korea. The host strain used for the isolation was P. aeruginosa (ATCC13388). An endolysin gene was annotated and named LysPA90 (GenBank accession no. MW815133).

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Hwang Y.J., Jo J., Kim E., Yoon H., Hong H., Kim M.S, & Myung H. (2022). Motility increase of adherent invasive Escherichia coli (AIEC) induced by a sub-inhibitory concentration of recombinant endolysin LysPA90. Frontiers in Microbiology, 13, 1093670.

Publication 2022

Escherichia coli ATCC8739

Bacteriophage Cultured media E coli Endolysin Gene Isolation Neut P aeruginosa Strain

Corresponding Organization : Hankuk University of Foreign Studies

Other organizations : Ajou University

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Variable analysis

independent variables

Bacteriophage PBPA90 infecting P. aeruginosa

dependent variables

Isolation and annotation of the endolysin gene LysPA90

control variables

Luria-Bertani (LB) media for culturing all strains
P. aeruginosa (ATCC13388) as the host strain used for the isolation of bacteriophage PBPA90

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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