RPMI8226, HEK293, and Wnt3a-L cells were purchased from the American Type Culture Collection and cultured in RPMI1640 (RPMI8226 and MM.1S) and Dulbecco’s modified Eagle’s media (HEK293 and Wnt3a-L) supplemented with 10% fetal bovine serum, 120 μg/ml penicillin, and 200 μg/ml streptomycin. HEK293-FL (TOPFlash) reporter cells were established as previously described [13 (link)]. Wnt3a-CM was prepared as previously described [14 (link)]. Luciferase assays were conducted using the Dual-Luciferase Assay Kit (Promega, USA), following the manufacturer’s instructions. Lithium chloride (LiCl), MG-132, and ursolic acid were purchased from Sigma-Aldrich (USA).
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