Influenza Virus Detection by RT-PCR

RNA was extracted from nasal wash as previously described [32 (link)]. A total of 4 µL RNA was assayed by real-time RT-PCR with influenza virus–specific primers and probes, using the SensiFAST Probe Lo-ROX One-Step Kit, according to the manufacturer's instructions (Bioline), on the 7500 Fast Real-Time system (Applied Biosystems). Primers and probes were from the Centers for Disease Control and Prevention (CDC) Influenza Virus Real-Time RT-PCR Influenza A (H1/H3/H1pdm09) Subtyping Panel and the CDC Influenza B Lineage Genotyping Panel, obtained from the Influenza Reagent Resource (available at: http://www.influenzareagentresource.org/).

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Laurie K.L., Guarnaccia T.A., Carolan L.A., Yan A.W., Aban M., Petrie S., Cao P., Heffernan J.M., McVernon J., Mosse J., Kelso A., McCaw J.M, & Barr I.G. (2015). Interval Between Infections and Viral Hierarchy Are Determinants of Viral Interference Following Influenza Virus Infection in a Ferret Model. The Journal of Infectious Diseases, 212(11), 1701-1710.

Publication 2015

SensiFAST Probe Lo-ROX One-Step Kit 7500 Fast Real-Time system

Influenza Influenza virus Influenza virus a Nasal Primers Real time pcr

Corresponding Organization : Victorian Infectious Diseases Reference Laboratory

Other organizations : University of Melbourne, York University, Royal Children's Hospital, Murdoch Children's Research Institute

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Variable analysis

independent variables

RNA extraction method (as previously described in [32])

dependent variables

Influenza virus detection and subtyping by real-time RT-PCR

control variables

Influenza virus-specific primers and probes used
SensiFAST Probe Lo-ROX One-Step Kit used for RT-PCR
7500 Fast Real-Time system (Applied Biosystems) used for RT-PCR

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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