Determination of hemolytic activity followed the method described by Reuben et al. (23 (link)). After culturing overnight in MRS broth, the isolated strain was streaked on sheep blood agar plates (Oxoid, Germany) and incubated at 37°C for 48 h. Blood lysis zones that appeared around the bacterial colonies were considered to indicate that the strains were hemolytic (β-hemolysis), while green-hued zones (α-hemolysis) or lack of any zones (γ-hemolysis) appearing around the bacterial colonies were considered to indicate that the strains were non-hemolytic.
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