Cell Lines and Virus Maintenance for IBDV Research

Human embryonic kidney (HEK) 293T cells (referred to as 293T cells) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (F2442-500ML; Sigma, St. Louis, MO, USA). Chicken embryo fibroblasts (DF-1) were grown in DMEM containing 10% FBS (CCS30010.02; MRC, QLD, Australia). The T7 RNA polymerase stably expressing cell line, BSRT7 (kindly gifted by Jingjing Cao, Shandong University, China), was cultured in DMEM containing 10% FBS (F2442-500ML; Sigma, St. Louis, MO, USA) and 500 μg/ml G418 (A100859; Sangon Biotech, Shanghai, China). Avibirnavirus IBDV strain NB is maintained in our laboratory (27 (link)).