The four commonly analyzed TSNA – NNN, NNK, Nʹ-nitrosoanatabine (NAT) and Nʹ-nitrosoanabasine (NAB) – were analyzed by liquid chromatography (LC)-tandem mass spectrometry (MS/MS) in positive ion electrospray mode as previously described.18 (link) Briefly, internal standards [13C6]NNN and [pyridine-D4]NNK were added to either cigarette filler samples or smoke filter pads, followed by extraction with citrate-phosphate buffer and purification of the extracts on ChemElut cartridges (Varian, Harbor City, California, USA) and Sep-Pak Plus silica cartridges (Waters, Milford, Massachusetts, USA). The purified samples were analyzed by LC-MS/MS in selected reaction monitoring mode as described.18 (link)
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Quantification of Tobacco-Specific Nitrosamines
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Buffer
Citrate
Liquid chromatography
Mass spectrometry
N nitrosoanabasine
N nitrosoanatabine
Phosphate
Pyridine
Silica
Smoke
Tandem mass spectrometry
Corresponding Organization : University of Minnesota
Other organizations : UPMC Hillman Cancer Center, University of Pittsburgh, Centers for Disease Control and Prevention, Shanghai Cancer Institute
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Variable analysis
independent variables
- Addition of internal standards [13C6]NNN and [pyridine-D4]NNK to cigarette filler samples or smoke filter pads
- Levels of the four commonly analyzed TSNA - NNN, NNK, Nʹ-nitrosoanatabine (NAT) and Nʹ-nitrosoanabasine (NAB) - measured by liquid chromatography (LC)-tandem mass spectrometry (MS/MS) in positive ion electrospray mode
- Extraction with citrate-phosphate buffer
- Purification of the extracts on ChemElut cartridges and Sep-Pak Plus silica cartridges
- Analysis by LC-MS/MS in selected reaction monitoring mode
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