Rat anti-mouse IL-17A (Clone #50104, catalog #MAB421) or IgG2A isotype control (Clone #54447, catalog #MAB006) antibody (R&D Systems, Inc., Minneapolis, MN) was administered weekly via intra-peritoneal injections (200μg/mouse, resuspended in 500μl sterile phosphate buffered saline [PBS]) starting 2 weeks post-HCT.19 (link)–21 (link) Based on pilot studies (data not shown), earlier and higher doses of anti-IL-17A were avoided due to concerns over potential impairment in hematopoietic cell engraftment.
Protocol detail
Anti-IL-17A Therapy Post-Hematopoietic Cell Transplant
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Antibody
Cell
Clone
Hematopoietic
Igg2a
Il 17a
Intra peritoneal injections
Isotype
Mouse
Phosphate
Saline
Sterile
Corresponding Organization : Duke University
Other organizations : Tulane University, East Carolina University
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Variable analysis
independent variables
- Administration of rat anti-mouse IL-17A (Clone #50104, catalog #MAB421) antibody
- Administration of IgG2A isotype control (Clone #54447, catalog #MAB006) antibody
- Outcomes measured after intra-peritoneal injections
- Sterile phosphate buffered saline (PBS) used to resuspend antibodies
- Injection volume of 500 μl per mouse
- Timing of injections (weekly, starting 2 weeks post-HCT)
- Positive control: Rat anti-mouse IL-17A (Clone #50104, catalog #MAB421) antibody
- Negative control: IgG2A isotype control (Clone #54447, catalog #MAB006) antibody
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