PANC-1 human pancreatic cancer cells were maintained in high-glucose DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Cells were pelleted, re-suspended in culture medium supplemented with 2% agarose and loaded into fluorinated ethylene-propylene (FEP) tubing suspended in a water bath (Fig. 1 ). Cells were exposed to 1 mM sodium cyanide to induce known metabolic perturbations, with images taken every 3 minutes.
Protocol detail
Inducing Metabolic Perturbations in Pancreatic Cancer Cells
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Agarose
Bath
Cells
Culture medium
Fetal bovine serum
Fluorinated ethylene propylene
Glucose
Human
Pancreatic cancer
Penicillin
Sodium cyanide
Streptomycin
Corresponding Organization : Smith-Kettlewell Eye Research Institute
Other organizations : University of Göttingen, Optica
Top 5 similar protocols
Variable analysis
independent variables
- Exposure to 1 mM sodium cyanide
- Metabolic perturbations
- High-glucose DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin
- Cells suspended in culture medium supplemented with 2% agarose and loaded into fluorinated ethylene-propylene (FEP) tubing
- Cells maintained in a water bath
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!