15N Labeling of Plant Nitrogen Uptake

Seeds of WT, OX and osasn1 plants were germinated and seedlings were cultured for 7 d in Yoshida nutrient solution (Yoshida et al. 1976) , in which 1.44 mM NH₄NO₃ was replaced by 2.88 mM NH₄Cl. Next, seedlings were treated with ¹⁵N-labeled NH₄Cl (10% atom ¹⁵NH₄Cl; Sigma-Aldrich). Roots and shoots were harvested at 12, 24, 36 and 48 h after treatment and dried at 70°C. The dried samples were weighed and ground, and ¹⁵N abundance was estimated as described previously (Masclaux-Daubresse and Chardon 2011) (link).

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Lee S., Park J., Lee J., Shin D., Marmagne A., Lim P.O., Masclaux-Daubresse C., An G, & Nam H.G. (2020). OsASN1 Overexpression in Rice Increases Grain Protein Content and Yield under Nitrogen-Limiting Conditions. Plant and Cell Physiology, 61(7), 1309-1320.

Publication 2020

15N-labeled NH4Cl

After treatment Nutrient Plants Roots Seedlings Seeds

Corresponding Organization :

Other organizations : Institute for Basic Science, Rural Development Administration, Institut Jean-Pierre Bourgin, Daegu Gyeongbuk Institute of Science and Technology, Kyung Hee University

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Variable analysis

independent variables

Genotype (WT, OX, osasn1)

dependent variables

15N abundance in roots and shoots
Dry weight of roots and shoots

control variables

Culture medium (Yoshida nutrient solution)
Incubation duration (12, 24, 36, 48 h)
15N-labeled NH4Cl (10% atom 15NH4Cl)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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