Cell Lines for PARP Inhibitor Resistance

UWB1.289 and UWB1.289 + BRCA1 were obtained from American Type Culture Collection (ATCC) and maintained in RPMI1640 (ATCC) and MEGM bullet kit (1:1; Lonza) with 3% FBS and 1% penicillin/streptomycin (DelloRusso et al. 2007 (link)). SYr-resistant cell lines were derived from the parental UWB1.289 line after 45 d of selection with 1.0 µM PARPi (olaparib; SelleckChem) or following passages with incremental increases of PARPi (olaparib) from 0.025 to 1.0 µM. T2 (non-BRCA), BR5 (BRCA1^Δ11/Δ11, exon 11 deleted), and resistant BR5-R1 cell lines are FVB mouse-derived ovarian tumor cell lines (Xing and Orsulic 2006 (link)) and were maintained in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, and 1% L-glutamine. BR5-R1 was derived through incremental increases of PARPi (olaparib) from 0.025 to 1.0 µM. HCC1937 and HCC1937 + BRCA1 cells were maintained in RPMI-1640 supplemented with 10% FBS and 1% penicillin/streptomycin. RPE-hTERT cells were maintained in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, and 1% L-glutamine. Primary human ovarian tumor cells were collected from ascites or pleural fluid of ovarian cancer patients that had tested positive for malignant cells by cytological analysis. Human ovarian tumor cells were maintained in RPMI-1640 with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, and supernatant from patient-matched ascites/pleural fluid. All cells were grown at 37°C and 5% CO₂.