Formalin-fixed, paraffin-embedded mouse livers were cut and stained with hematoxylin and eosin (H&E) or Picrosirius Red (Abcam) dyes. Liver histology was blindly scored by an independent histopathologist based on three semiquantitative items: steatosis (0–3), lobular inflammation (0–3) and hepatocellular ballooning (0–2) (not shown) (Liang et al., 2014 (link)). Stained sections were scanned with NanoZoomer Digital slide scanner (Hamamatsu) and quantification of Picrosirius Red-stained areas was performed using ImageJ on three independent areas per section. Data is represented as the average positively-stained percent of area of interest.
Hepatic macrophage content was assessed by quantification of F4/80 positively-stained areas (ab6640, Abcam).
Hepatic macrophage content was assessed by quantification of F4/80 positively-stained areas (ab6640, Abcam).
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