Western Blotting Analysis of BMP4 and GPX4 Proteins

Western blotting was performed according to a previously described procedure [26 (link)]. The following antibodies were used to test the expression of protein: anti-BMP4 (EPR6211) (ab124714, Abcam), anti-BMP4 (ab39973, Abcam), and anti-glutathione peroxidase 4 (anti-GPX4) (ab125066, Abcam). Anti-GAPDH (#5174, Cell Signaling Technology) was used as an internal loading control. Experiments were repeated more than three times, and representative images are presented in the article at the end.

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Wang X., Ma B., Wen X., You H., Sheng C., Bu L, & Qu S. (2022). Bone morphogenetic protein 4 alleviates nonalcoholic steatohepatitis by inhibiting hepatic ferroptosis. Cell Death Discovery, 8, 234.

Publication 2022

ab39973 ab125066 Anti-GAPDH

Antibodies Bmp4 Gapdh Glutathione peroxidase 4 Protein

Corresponding Organization :

Other organizations : Shanghai Tenth People's Hospital, Tongji University

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Variable analysis

independent variables

Anti-BMP4 (EPR6211) (ab124714, Abcam)
Anti-BMP4 (ab39973, Abcam)
Anti-glutathione peroxidase 4 (anti-GPX4) (ab125066, Abcam)

dependent variables

Expression of protein

control variables

Anti-GAPDH (#5174, Cell Signaling Technology)

controls

Positive control: not mentioned
Negative control: not mentioned

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