Natrium benzoate (100 mg/kg, Sigma-Aldrich, St. Louis, MO, United States) was dissolved in 100 μL of water and the rats were treated with NaB-containing water via gavage at 1 h after t-SCI (Khasnavis and Pahan, 2014 (link); Kundu et al., 2016 (link)). An Akt inhibitor, MK2206, (100 μg, Selleck Chemicals, Houston, TX, United States) was dissolved in dimethyl sulfoxide and further diluted in 10 μL of sterile saline. The rats were treated with MK2206-containing normal saline via intrathecal injections at 1 h after t-SCI (Yan et al., 2017 (link)).
Two target-specific siRNAs disturbing rat DJ-1 mRNA mixtures (sense: 5’-CCCAUUGGCUAAGGACAAATT-3’, 5’-UGGAGACGGUCAUCCCUGUTT-3’) or scramble siRNA (sense: 5’-UUCUCCGAACGUGUCACGUTT-3’) obtained from Thermo Fisher Scientific (Waltham, MA, United States) were dissolved in EntransterTM-in vivo transfection reagent (500 pmol/10 μL, Engreen Biosystem, Beijing, China). The rats were intrathecally injected with siRNA solution at 48 h before t-SCI as previously described (Figueroa et al., 2016 (link)).
Two target-specific siRNAs disturbing rat DJ-1 mRNA mixtures (sense: 5’-CCCAUUGGCUAAGGACAAATT-3’, 5’-UGGAGACGGUCAUCCCUGUTT-3’) or scramble siRNA (sense: 5’-UUCUCCGAACGUGUCACGUTT-3’) obtained from Thermo Fisher Scientific (Waltham, MA, United States) were dissolved in EntransterTM-in vivo transfection reagent (500 pmol/10 μL, Engreen Biosystem, Beijing, China). The rats were intrathecally injected with siRNA solution at 48 h before t-SCI as previously described (Figueroa et al., 2016 (link)).
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