Erythrocyte Membrane Fatty Acid Analysis

An aliquot of the blood samples (250 μL) used for the HDFM measurements was then used for the separation of the blood cells, isolation of mature RBC membrane phospholipids and examination of fatty acid composition, applying previously published protocols^{7 (link)}. We evaluated the fatty acids of the mature erythrocyte membrane phospholipids as relative percentages (% rel) referred to a cluster of 12 fatty acids (10 cis and 2 trans fatty acids)². The mean % rel ± standard deviation (sd) of the RBC membrane fatty acid cluster for healthy and ASD children with the decreased value of DHA in the ASD children (P = 0.0424) are reported in the Supplementary Table 4.

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Giacometti G., Ferreri C., Sansone A., Chatgilialoglu C., Marzetti C., Spyratou E., Georgakilas A.G., Marini M., Abruzzo P.M., Bolotta A., Ghezzo A., Minguzzi R., Posar A, & Visconti P. (2017). High predictive values of RBC membrane-based diagnostics by biophotonics in an integrated approach for Autism Spectrum Disorders. Scientific Reports, 7, 9854.

Publication 2017

Blood Blood cells Children Erythrocyte membrane Fatty acid Isolation Membrane Phospholipids Trans fatty acids

Corresponding Organization :

Other organizations : Institute of Organic Synthesis and Photoreactivity, National Technical University of Athens, University of Bologna, Institute of Neurological Sciences

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Relative percentages (% rel) of the fatty acids of the mature erythrocyte membrane phospholipids, referred to a cluster of 12 fatty acids (10 cis and 2 trans fatty acids)

control variables

None explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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