Generation and Characterization of PARP1/2 KO Mice

PARP1 and PARP2 global KO mice, as well as PARP2 flox mice were provided by Dr. Francoise Dantzer^{5 (link),19 (link),27 (link)}. Generation of PARP1 flox mice has been published^{28 (link)}. To establish conditional PARP1 or PARP2 KO in the myeloid lineage, PARP1 or PARP2 flox mice were bred with LyzM-Cre mice^{29 (link)}. MMTV-PyMT transgenic C57BL/6 mice and MMTV-Cre mice were from the Jackson Laboratory. WT C57BL/6 J mice (UTSW Breeding Core), BALB/cJ mice (UTSW Breeding Core), and athymic nude mice (Charles River) were also used. Nude PARP1 and PARP2 global KO mice were established by breeding PARP1 and PARP2 global KO C57BL/6 mice with athymic nude mice. All experiments were conducted using littermates. All protocols for mouse experiments were approved by the Institutional Animal Care and Use Committee of University of Texas Southwestern Medical Center. All mice were housed in accordance with approved IACUC protocols. Animals were housed on a 12–12 light cycle (light on 6:00, off 18:00) and provided food and water ad libitum.