Nasopharyngeal Squamous Carcinoma Cell Lines

NPC076 and NPC039 are the isolated nasopharyngeal squamous carcinoma cell lines used in this study [10 (link)]. The two cell lines were cultured in DMEM/F-12 (Invitrogen, Carlsbad, CA, USA) supplemented with 5% FBS at 37 °C under 5% CO₂/95% air. The cell lines were incubated and conditioned with the following reagents for experiment control: Z-ATAD-fmk (1–3 μM, BioVision, San Francisco, CA, USA), a cell permeable caspase 12 inhibitor. BMS345541 (1–5 μM, #16667, Cayman, Ann Arbor, MI, USA), a cell permeable IKKα/β inhibitor. Multiple antibodies were purchased and applied for either immunofluorescence staining, western blotting, or coimmunoprecipitation. Antibodies for a variety of proteins are listed as follows: antibody for Casp12 (#55238-1-AP, Proteintech, Rosemont, IL, USA), IκBα, IKKα’IKKβ, p-IκBα, and p-p65 (#4812, #2682, #8943s, #9246, #3033, Cell Signaling, Danvers, MA, USA), IKKα, NEMO (#sc14A231, #sc8032, Santa Cruz, Dallas, Texas, USA), or GFP tags (#GTX628528, GeneTex, Hsinchu City, Taiwan). P-IkB Human Casp12 (hCasp12), Casp12 (GFP-tagged), is from the human Casp12 cDNA (#RG231175, OriGene, Rockville, MD, USA).