Whole yeast protein extracts were obtained as described [82 (link)]. Actin and tubulin were visualized on an 8% SDS-PAGE with the anti-actin antibody (Thermo Scientific, Waltham, MA, USA) or anti-α-tubulin antibody (Santa Cruz Biotechnology, Dallas, TX, USA). The densitometry of the bands was determined using Image Lab 6.1 (BioRad, Hercules, CA, USA) and quantification using GraphPad Prism8 (Graph-Pad Software, Inc., Boston, MA, USA).
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