Culturing EBV-Positive and Negative Cell Lines

EBV-negative HSC1 and SCC25 cells, as well as EBV-positive HSC1-EBV and SCC25-EBV cells [6 (link)], were cultured in Dulbecco’s Modified Eagle Medium (DMEM)/Ham’s F-12 (Wako, Osaka, Japan) supplemented with 10% fetal bovine serum (FBS) (HyClone^TM, GE Healthcare Life Sciences, Logan, UT, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Nacalai Tesque Inc., Kyoto, Japan). The cells were cultured at 37 °C in a humidified incubator with 5% CO₂.

Free full text: Click here

Heawchaiyaphum C., Yoshiyama H., Iizasa H., Burassakarn A., Tumurgan Z., Ekalaksananan T, & Pientong C. (2023). Epstein–Barr Virus Promotes Oral Squamous Cell Carcinoma Stemness through the Warburg Effect. International Journal of Molecular Sciences, 24(18), 14072.

Publication 2023

DMEM)/Ham’s F-12 HyCloneTM penicillin streptomycin

Cells Cultured medium Eagle Fetal bovine serum Penicillin Streptomycin

Corresponding Organization : Khon Kaen University

Other organizations : Thammasat University

Top 5 similar protocols

Variable analysis

independent variables

EBV status (EBV-negative vs. EBV-positive)

dependent variables

Not explicitly mentioned

control variables

Cell lines (HSC1 and SCC25)
Culture medium (DMEM/Ham's F-12 supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin)
Incubation conditions (37 °C, 5% CO2, humidified)

controls

EBV-negative HSC1 and SCC25 cells as negative controls
EBV-positive HSC1-EBV and SCC25-EBV cells as positive controls

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!