Western Blot Analysis of Synaptic Proteins

For western blotting, the procedure was performed as previous study at the age of 6 months [25 (link)]. Analysis of the data was performed using NIH ImageJ software, and the mean density of each band was normalized to β-actin signal in the same sample and averaged. For primary antibodies, we used mouse anti-β-actin (1:3000, Thermo, MA5–15739), rabbit anti-Tau (phosphor-S396) (1:1000, Abcam, ab109390), mouse anti-GluA1 (1:1000, Santa Cruz, sc-13,152), goat anti-GluA2 (1:200, Santa Cruz, sc-7611), mouse anti-GluN1 (1:1000, BD, 556308), rabbit anti-GluN2A (1:1000, Millipore, ab1555P) mouse anti-GluN2B (1:1000, BD, 610417).

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Ai P.H., Chen S., Liu X.D., Zhu X.N., Pan Y.B., Feng D.F., Chen S., Xu N.J, & Sun S. (2020). Paroxetine ameliorates prodromal emotional dysfunction and late-onset memory deficit in Alzheimer’s disease mice. Translational Neurodegeneration, 9, 18.

Publication 2020

mouse anti-β-actin mouse anti-GluA1 mouse anti-GluN1 mouse anti-GluN2B

Actin Antibodies Glun2a Glun2b Goat Mouse Phosphor Rabbit

Corresponding Organization :

Other organizations : Shanghai Jiao Tong University, Ruijin Hospital, Shanghai Center for Brain Science and Brain-Inspired Technology, Shanghai Ninth People's Hospital

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Variable analysis

independent variables

Age of the subjects (6 months)

dependent variables

Phosphorylation of Tau at S396
Levels of GluA1, GluA2, GluN1, GluN2A, and GluN2B proteins

control variables

Western blotting procedure from a previous study [25]
β-actin protein as a loading control

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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