MCF10A Cell Line Culture Protocol

MCF10A cell lines were obtained from American Type Culture Collection (ATCC) and cultured in Dulbecco's modified Eagle medium/F12 (DMEM/F12) supplemented with 5% Horse Serum (Invitrogen), 10 μg/ml insulin, 100 ng/ml Cholera toxin (Sigma Aldrich), 20 ng/ml Epidermal Growth Factor, 1% Penicillin-Streptomycin (Cellgro), and 0.5 μg/ml Hydrocortisone. All cells were grown at 37°C in 5% CO₂. They were recently authenticated (Russ et al., 2012 (link)) and checked for contamination.

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Daniel S.G., Russ A.D., Guthridge K.M., Raina A.I., Estes P.S., Parsons L.M., Richardson H.E., Schroeder J.A, & Zarnescu D.C. (2017). miR-9a mediates the role of Lethal giant larvae as an epithelial growth inhibitor in Drosophila. Biology Open, 7(1), bio027391.

Publication 2017

MCF10A cell lines DMEM/F12 Horse Serum Cholera toxin Penicillin-Streptomycin

Cell lines Cells Cholera toxin Cultured medium Eagle Epidermal growth factor Horse Hydrocortisone Insulin Penicillin Serum Streptomycin

Corresponding Organization :

Other organizations : University of Arizona, Peter MacCallum Cancer Centre, University of Melbourne, Monash University, La Trobe University

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Culture medium (DMEM/F12 supplemented with 5% Horse Serum, 10 μg/ml insulin, 100 ng/ml Cholera toxin, 20 ng/ml Epidermal Growth Factor, 1% Penicillin-Streptomycin, and 0.5 μg/ml Hydrocortisone)
Incubation conditions (37°C in 5% CO2)

controls

Positive control: Not specified
Negative control: Not specified

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