Antibody Inhibition of G-CSFR in C. perfringens Infection

Mice were subcutaneously injected with 1.5 µg of filgrastim or the same volume of PBS, shortly after the injection of C. perfringens. To inhibit G-CSFR, a neutralizing antibody against G-CSFR (R&D systems, Minneapolis, MN, USA) was used as described in a previous report [33 (link)]. The neutralizing antibody or Rat IgG_2B isotype control (R&D systems, Minneapolis, MN, USA) was intraperitoneally administered to C. perfringens-infected mice at 5 µg per mouse shortly after the injection of C. perfringens. This dose was 10 times higher than that in the previous report [33 (link)].

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Takehara M., Sonobe Y., Bandou H., Kobayashi K, & Nagahama M. (2019). Granulocyte Colony-Stimulating Factor Does Not Influence Clostridium Perfringens α-Toxin-Induced Myonecrosis in Mice. Toxins, 11(9), 509.

Publication 2019

Rat IgG2B isotype control

Antibody g C perfringens Csfr Filgrastim Igg2b Inhibit Isotype Mouse Neutralizing antibody

Corresponding Organization : Tokushima Bunri University

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Variable analysis

independent variables

Filgrastim (1.5 µg)
PBS (same volume as filgrastim)

dependent variables

Not explicitly mentioned

control variables

Timing of injections (shortly after C. perfringens injection)

controls

Positive control: Neutralizing antibody against G-CSFR
Negative control: Rat IgG2B isotype control

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