The formation of HMMS of PF-06439535 was assessed by SE-HPLC. Isocratic elution (20 mM sodium phosphate, 400 mM sodium chloride, pH 6.0) of the samples was carried out using a YMC-Pack Diol-200 column (300 mm × 8 mm, pore size 200 Å, particle size 5 µm; YMC, Koyoto, Japan) on a Waters HPLC system (Waters Corporation, Milford, MA, USA) with ultraviolet (UV) detection and was performed at a wavelength of 280 nm.
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SE-HPLC analysis of PF-06439535 HMMs
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- Isocratic elution conditions (20 mM sodium phosphate, 400 mM sodium chloride, pH 6.0)
- Formation of HMMs of PF-06439535 assessed by SE-HPLC
- YMC-Pack Diol-200 column (300 mm × 8 mm, pore size 200 Å, particle size 5 µm)
- Waters HPLC system (Waters Corporation, Milford, MA, USA) with ultraviolet (UV) detection at 280 nm
- No positive or negative controls were explicitly mentioned in the input.
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