Histological Analysis of Lung Tissue

The deparaffinized lung sections were stained using SR solution (0.1% in aqueous saturated picric acid) for 1 h and differentiated in 0.5% acetic acid. Subsequently, the slides were counterstained with hematoxylin for 10 min to stain the cell nuclei as described by Xu et al. [32 (link)]. Increasing alcohol series and Roti-Histol® was used for dehydration of the sections that were then covered with Entellan®. One area per animal out of a total of 6 mice per group was analyzed for the evaluation of the trauma. This number is based on the power analysis conducted for the animal experiment application (license number: 1183). Pictures were taken with the UC30 color camera at X10 and X40 magnification. All stainings were acquired with an Olympus IX81 microscope and analyzed with the Olympus software cellSens Dimensions 2.3 (Build 18,987).